htert-adsc.strail cells (5 x 10 3 cells/well) (Becton Dickinson)

Becton Dickinson htert-adsc.strail cells (5 x 10 3 cells/well)

The <t>sTRAIL-overexpressing</t> <t>hTERT</t> immortalized human adipose stem cell line <t>(hTERT-ADSC.</t> sTRAIL). (A) hTERT-ADSC and hTERT-ADSC.sTRAIL were generated by the lentiviral transduction of GFP and the sTRAIL gene using the CLV-Ubic vector. (B), (C): The expression of the sTRAIL transcript or protein was confirmed by RT-PCR or Western blots, respectively. (D) Phase-contrast microscopy of the hTERT-ADSC.GFP line (left) and hTERT-hADSC.GFP. sTRAIL (right) line. ADCS = hTERT-hADSC, sTRAIL = secretable trimeric forms of tumor necrosis factor (TNF)-related apoptosis-inducing ligand, ADSC = adipose-derived stem cells, ADSC.sTRAIL = sTRAIL-overexpressing ADSC, RT-PCR = reverse transcription-polymerase chain reaction.

Htert Adsc.Strail Cells (5 X 10 3 Cells/Well), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The sTRAIL-overexpressing hTERT immortalized human adipose stem cell line (hTERT-ADSC. sTRAIL). (A) hTERT-ADSC and hTERT-ADSC.sTRAIL were generated by the lentiviral transduction of GFP and the sTRAIL gene using the CLV-Ubic vector. (B), (C): The expression of the sTRAIL transcript or protein was confirmed by RT-PCR or Western blots, respectively. (D) Phase-contrast microscopy of the hTERT-ADSC.GFP line (left) and hTERT-hADSC.GFP. sTRAIL (right) line. ADCS = hTERT-hADSC, sTRAIL = secretable trimeric forms of tumor necrosis factor (TNF)-related apoptosis-inducing ligand, ADSC = adipose-derived stem cells, ADSC.sTRAIL = sTRAIL-overexpressing ADSC, RT-PCR = reverse transcription-polymerase chain reaction.

Journal: Prostate International

Article Title: Enhanced inhibition of tumor growth using TRAIL-overexpressing adipose-derived stem cells in combination with the chemotherapeutic agent CPT-11 in castration-resistant prostate cancer

doi: 10.1016/j.prnil.2020.07.002

Figure Lengend Snippet: The sTRAIL-overexpressing hTERT immortalized human adipose stem cell line (hTERT-ADSC. sTRAIL). (A) hTERT-ADSC and hTERT-ADSC.sTRAIL were generated by the lentiviral transduction of GFP and the sTRAIL gene using the CLV-Ubic vector. (B), (C): The expression of the sTRAIL transcript or protein was confirmed by RT-PCR or Western blots, respectively. (D) Phase-contrast microscopy of the hTERT-ADSC.GFP line (left) and hTERT-hADSC.GFP. sTRAIL (right) line. ADCS = hTERT-hADSC, sTRAIL = secretable trimeric forms of tumor necrosis factor (TNF)-related apoptosis-inducing ligand, ADSC = adipose-derived stem cells, ADSC.sTRAIL = sTRAIL-overexpressing ADSC, RT-PCR = reverse transcription-polymerase chain reaction.

Article Snippet: The hTERT-ADSC.sTRAIL cells (5 x 10 3 cells/well) were plated in 96-well plates (Falcon, Becton-Dickinson Co.).

Techniques: Generated, Transduction, Plasmid Preparation, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Microscopy, Derivative Assay

Cell markers of hTERT-ADSC.GFP and hTERT-ADSC.GFP.sTRAIL cells. hTERT-ADSC cells express cell type–specific markers for mesenchymal stem cell markers CD29, CD90, and CD105, but do not express CD34 or CD45 cell type markers for hematopoietic stem cells. ADSC = hTERT immortalized human adipose-derived stem cells, ADSC.sTRAIL = sTRAIL-overexpressing ADSCs.

Journal: Prostate International

doi: 10.1016/j.prnil.2020.07.002

Figure Lengend Snippet: Cell markers of hTERT-ADSC.GFP and hTERT-ADSC.GFP.sTRAIL cells. hTERT-ADSC cells express cell type–specific markers for mesenchymal stem cell markers CD29, CD90, and CD105, but do not express CD34 or CD45 cell type markers for hematopoietic stem cells. ADSC = hTERT immortalized human adipose-derived stem cells, ADSC.sTRAIL = sTRAIL-overexpressing ADSCs.

Article Snippet: The hTERT-ADSC.sTRAIL cells (5 x 10 3 cells/well) were plated in 96-well plates (Falcon, Becton-Dickinson Co.).

Techniques: Derivative Assay

hTERT-ADSC.sTRAIL line targets prostate cancer. (A), (B) The invasion study shows the migration of hTERT-ADSC or hTERT-ADSC.sTRAIL to prostate cancer. (C) Intracardiac delivery of hTERT-ADSC targeting prostate cancer. qPCR demonstrated GFP and human sTRAIL from experimentally induced prostate cancer tissues removed from the mice. (D) Expression of chemoattractant factors. The presence of the ligands, SCF, SDF-1, and VEGF in PC3 cells and their corresponding receptors c-kit, CXCR4, VEGFR1, VEGFR2, and VEGFR3 in hTERT-ADSC cells. ADCS = hTERT-ADSC, stem cell factor, SCF = stromal cell–derived factor, SDF = vascular endothelial growth factor, VEGF = vascular endothelial growth factor, VEGFR = vascular endothelial growth factor receptor, ADSC = hTERT immortalized human adipose stem cell line, ADSC.sTRAIL = sTRAIL-overexpressing ADSCs. ∗ P < 0.05.

Journal: Prostate International

doi: 10.1016/j.prnil.2020.07.002

Figure Lengend Snippet: hTERT-ADSC.sTRAIL line targets prostate cancer. (A), (B) The invasion study shows the migration of hTERT-ADSC or hTERT-ADSC.sTRAIL to prostate cancer. (C) Intracardiac delivery of hTERT-ADSC targeting prostate cancer. qPCR demonstrated GFP and human sTRAIL from experimentally induced prostate cancer tissues removed from the mice. (D) Expression of chemoattractant factors. The presence of the ligands, SCF, SDF-1, and VEGF in PC3 cells and their corresponding receptors c-kit, CXCR4, VEGFR1, VEGFR2, and VEGFR3 in hTERT-ADSC cells. ADCS = hTERT-ADSC, stem cell factor, SCF = stromal cell–derived factor, SDF = vascular endothelial growth factor, VEGF = vascular endothelial growth factor, VEGFR = vascular endothelial growth factor receptor, ADSC = hTERT immortalized human adipose stem cell line, ADSC.sTRAIL = sTRAIL-overexpressing ADSCs. ∗ P < 0.05.

Article Snippet: The hTERT-ADSC.sTRAIL cells (5 x 10 3 cells/well) were plated in 96-well plates (Falcon, Becton-Dickinson Co.).

Techniques: Migration, Expressing, Derivative Assay

In vitro effect of combined treatment with hTERT-ADSC.sTRAIL and cpt-11. (A) The suicide effect. At greater than 1 μM CPT-11, the viability of ADSC.sTRAIL cells was lower than at more than 0 μM CPT-11 ( P < 0.05). (B) The cytotoxicity of ADSC.sTRAIL and 5 μM CPT-11 toward PC3 at cocultured ratios of 0.05. The cell viability of PC3 decreased significantly by treatment with both CPT-11 and ADSC.sTRAIL compared with CPT-11 treatment alone. (D) Flow cytometry for propidium iodide/annexin V staining showed that the percentage of annexin V–positive apoptotic PC3 cells increased in the presence of ADSC.sTRAIL and CPT-11 compared to CPT-11 treatment alone. (E) The percentage of apoptotic cells significantly increased in the ADSC.sTRAIL-treated group compared with those treated with cpt-11 alone ( P < 0.05). ADSC = hTERT immortalized human adipose-derived stem cells, ADSC.sTRAIL = sTRAIL-overexpressing hTERT immortalized human adipose stem cells. ∗ P < 0.05.

Journal: Prostate International

doi: 10.1016/j.prnil.2020.07.002

Figure Lengend Snippet: In vitro effect of combined treatment with hTERT-ADSC.sTRAIL and cpt-11. (A) The suicide effect. At greater than 1 μM CPT-11, the viability of ADSC.sTRAIL cells was lower than at more than 0 μM CPT-11 ( P < 0.05). (B) The cytotoxicity of ADSC.sTRAIL and 5 μM CPT-11 toward PC3 at cocultured ratios of 0.05. The cell viability of PC3 decreased significantly by treatment with both CPT-11 and ADSC.sTRAIL compared with CPT-11 treatment alone. (D) Flow cytometry for propidium iodide/annexin V staining showed that the percentage of annexin V–positive apoptotic PC3 cells increased in the presence of ADSC.sTRAIL and CPT-11 compared to CPT-11 treatment alone. (E) The percentage of apoptotic cells significantly increased in the ADSC.sTRAIL-treated group compared with those treated with cpt-11 alone ( P < 0.05). ADSC = hTERT immortalized human adipose-derived stem cells, ADSC.sTRAIL = sTRAIL-overexpressing hTERT immortalized human adipose stem cells. ∗ P < 0.05.

Article Snippet: The hTERT-ADSC.sTRAIL cells (5 x 10 3 cells/well) were plated in 96-well plates (Falcon, Becton-Dickinson Co.).

Techniques: In Vitro, Flow Cytometry, Staining, Derivative Assay

Treatment with hTERT-hADSC.sTRAIL cells and CPT-11 has a significant therapeutic effect in vivo . (A) The schematic summary of the treatment. (B) Illustration of the induction of prostate cancer using PC3 cells, the systemic injection of hTERT-ADSC.sTRAIL cells, and the migration of gene-modified stem cells toward prostate cancer. Blue: PC3; red: hTERT-ADSC.sTRAIL cells. (C) Administration of hTERT-hADSC.sTRAIL in combination with 1.7 mg/kg/day CPT-11 reduced prostate cancer tumor volumes in mice. The inhibitory effects of hTERT-ADSC.sTRAIL combined with CPT-11 were higher than those of hTERT-ADSC.sTRAIL or cpt-11 alone. (D) The inhibitory effects of hTERT-ADSC.CE combined with a high CPT-11 concentration (13.5 mg/kg/day) were better than those at a low CPT-11 concentration (17 mg/k/day). ADSC = hTERT immortalized human adipose stem cell line, hTERT-ADSC.sTRAIL = sTRAIL-overexpressing hTERT immortalized human adipose stem cells, H&E = hematoxylin & eosin. ∗ P < 0.05.

Journal: Prostate International

doi: 10.1016/j.prnil.2020.07.002

Figure Lengend Snippet: Treatment with hTERT-hADSC.sTRAIL cells and CPT-11 has a significant therapeutic effect in vivo . (A) The schematic summary of the treatment. (B) Illustration of the induction of prostate cancer using PC3 cells, the systemic injection of hTERT-ADSC.sTRAIL cells, and the migration of gene-modified stem cells toward prostate cancer. Blue: PC3; red: hTERT-ADSC.sTRAIL cells. (C) Administration of hTERT-hADSC.sTRAIL in combination with 1.7 mg/kg/day CPT-11 reduced prostate cancer tumor volumes in mice. The inhibitory effects of hTERT-ADSC.sTRAIL combined with CPT-11 were higher than those of hTERT-ADSC.sTRAIL or cpt-11 alone. (D) The inhibitory effects of hTERT-ADSC.CE combined with a high CPT-11 concentration (13.5 mg/kg/day) were better than those at a low CPT-11 concentration (17 mg/k/day). ADSC = hTERT immortalized human adipose stem cell line, hTERT-ADSC.sTRAIL = sTRAIL-overexpressing hTERT immortalized human adipose stem cells, H&E = hematoxylin & eosin. ∗ P < 0.05.

Article Snippet: The hTERT-ADSC.sTRAIL cells (5 x 10 3 cells/well) were plated in 96-well plates (Falcon, Becton-Dickinson Co.).

Techniques: In Vivo, Injection, Migration, Modification, Concentration Assay

Immunohistochemistry of removed prostate cancer tumors. (A)–(D) H&E staining of induced prostate cancer two weeks after the implantation of PC3 cells into the flanks of the mice. (A) Tumors in PC3-transplanted mice (X100), Inset: (X400). The tumor is well-demarcated from the dermal tissue and composed of closely packed cancer cells. (B) Tumors in PC3-transplanted mice after 13.5 mg/kg/day CPT-11 systemic treatment (X100). (C) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL systemic treatment (X100). (D) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL combined with 13.5 mg/kg/day CPT-11 systemic treatment (X100). Geographic necrosis was seen. The neoplastic cells remaining in the peripheral lesion were shrunken and had distinct borders. (E), (F) TRAIL immunohistochemical staining of tumors after treatment shows anti-TRAIL–positive cells. (E) Tumors in the PC3-transplanted mice after hTERT-ADSC.sTRAIL systemic treatment (X100), Inset: (X400). (F) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL combined with 13.5 mg/kg/day CPT-11 systemic treatment (X100), Inset: (X400) (G), (H) TUNEL immunohistochemical staining of tumors after treatment shows TUNEL-positive cells. (G) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL systemic treatment (X100), Inset: (X400). (H) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL combined with 13.5 mg/kg/day CPT-11 systemic treatment (X100), Inset: (X400). Hyperchromatic nuclei are centrally located with condensed eosinophilic cytoplasm, which have apoptotic bodies. Inset: Apoptotic cells with apoptotic bodies. Bar. 500 μm. H&E = hematoxylin & eosin, TUNEL = terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling.

Journal: Prostate International

doi: 10.1016/j.prnil.2020.07.002

Figure Lengend Snippet: Immunohistochemistry of removed prostate cancer tumors. (A)–(D) H&E staining of induced prostate cancer two weeks after the implantation of PC3 cells into the flanks of the mice. (A) Tumors in PC3-transplanted mice (X100), Inset: (X400). The tumor is well-demarcated from the dermal tissue and composed of closely packed cancer cells. (B) Tumors in PC3-transplanted mice after 13.5 mg/kg/day CPT-11 systemic treatment (X100). (C) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL systemic treatment (X100). (D) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL combined with 13.5 mg/kg/day CPT-11 systemic treatment (X100). Geographic necrosis was seen. The neoplastic cells remaining in the peripheral lesion were shrunken and had distinct borders. (E), (F) TRAIL immunohistochemical staining of tumors after treatment shows anti-TRAIL–positive cells. (E) Tumors in the PC3-transplanted mice after hTERT-ADSC.sTRAIL systemic treatment (X100), Inset: (X400). (F) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL combined with 13.5 mg/kg/day CPT-11 systemic treatment (X100), Inset: (X400) (G), (H) TUNEL immunohistochemical staining of tumors after treatment shows TUNEL-positive cells. (G) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL systemic treatment (X100), Inset: (X400). (H) Tumors in PC3-transplanted mice after hTERT-ADSC.sTRAIL combined with 13.5 mg/kg/day CPT-11 systemic treatment (X100), Inset: (X400). Hyperchromatic nuclei are centrally located with condensed eosinophilic cytoplasm, which have apoptotic bodies. Inset: Apoptotic cells with apoptotic bodies. Bar. 500 μm. H&E = hematoxylin & eosin, TUNEL = terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling.

Article Snippet: The hTERT-ADSC.sTRAIL cells (5 x 10 3 cells/well) were plated in 96-well plates (Falcon, Becton-Dickinson Co.).

Techniques: Immunohistochemistry, Staining, Immunohistochemical staining, TUNEL Assay, End Labeling

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